It combines multiple optimized features to provide powerful performance and reliable results that your research demands. For uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user. In this case, the software can be installed on the user s office computer. Outstanding thermal and optical performance due to rotary format an unmatched optical range spanning uv to infrared wavelengths stateofthe art analyses supported by user friendly software low maintenance and maximum convenience due to robust design high performance in multiple applications with.
Userfriendly software interface the rotorgene offers the most userfriendly realtime analysis software system currently available. The rotorgene q has multiple design features providing you with the outstanding performance and reliable results that your research demands. Rotorgene q mdx 5plex hrm user manual ca 052018 contents3 6. Qiagen rotor gene q 5plex real time pcr with hrm on sale. Qiagens realtime pcr cycler, the rotorgene q, combines multiple optimized design features to. Please refer to the rotorgene q user manual for complete information about the proper care, maintenance, and use of the rotorgene q cycler. If the rotorgene q instrument is used with kits other than qiagen kits, it is the user s responsibility to validate the performance of such product combination for any particular application. Qiagens realtime pcr cycler, the rotorgene q, combines multiple optimized design features to provide the outstanding performance and reliabl. The 36well rotor and 72well rotor for the rotorgene q are not to be used on rotorgene 3000 instruments due to optical alignment incompatibilities. Check the whatsnew section of this manual for an update on changes and novel features in this version of the software. Type in the serial number six digits, which is located at the back of the rotorgene.
Any suggestions for the rotor gene troubleshooting. User guide contractual use of its customer in connection with the use of the products option 3. Kasp chemistry for allelic discrimination performs well on a qiagen rotorgene q instrument and this stepby step protocol will enable users to successfully. This help manual will instruct the user through run setup and analysis. Qiagen rotor gene q 5plex hrm real time pcr youtube. Rotorgene q a rapid, automatable realtime pcr instrument for. For uptodate licensing information and productspecific disclaimers, see the respective qiagen kit handbook or user manual. Laboratory equipment qiagen rotorgene q mdx installation manual 24 pages laboratory equipment qiagen bluv user manual. Fda summary of safety and effectiveness data page 1 summary of safety and effectiveness data ssed i.
Qiagen rotor gene q software download titleqiagen rotor gene q software download easyartus rg pcr kits cewith easyartus rg pcr kits, qiagen now provides fully integrated automated sample purification and detection of bk virus, cmv, ebv, hsv, and vzv as ce ivd marked solutions, as required, for example, for routine transplantation diagnostics. The rotorgene q, if used in combination with qiagen kits indicated for use with the rotor. For neutralization of dna of dead organisms 44 pages. Which reads are countable depends on the user settings when the variant calling is performed if e. Qiagens realtime pcr cycler, the rotorgene q, combines multiple. The instructions and safety information in the user manual must be followed to ensure safe operation of the instrument and to maintain the instrument in a safe. Rotorgene q mdx user manual us 022018 contents3 1 operating procedures rotorgene assaymanager software 61 2 access protection 21 2. Lowabundance transcripts are reliably quantified, even when. Qiagen rotor gene q 5plex hrm real time pcr available at the lab world group. Do not use components of the kit that have passed their expiration date, or. Should there be an exceptional situation in which we process personal data for the performance of a task carried out in the public interest or in the exercise of official authority vested in us or for reasons necessary for the legitimate interests of our or third parties, you have a right to object to such processing of your personal data, including profiling based on these reasons, and we. The optimized kit listed for gene expression at qiagen website is as following for both onestep and twostep pcr. Qiagen rtpcr kit rotor gene q which one should i use.
Rotor gene q mdx user manual, revision r3, for rotor gene q mdx instruments using rotor gene q software version 2. The rotorgene q, if used in combination with qiagen kits indicated for use with the rotorgene q instrument, is intended for the applications described in the respective qiagen kit handbooks. Page option determines how the reaction data are to be grouped for analysis purposes after import into the rotorgene q software. Fixed optical path length led excites from the side of the chamber. Print bookmark share for rotorgene q instruments using rotorgene q software version 2. Qiagen is the leading provider of innovative sample and assay technologies.
In the rotorgene q software, tm is typically determined by assigning the minimum of the corresponding inverted first derivative curve for details, consult the rotorgene q user manual. Guide to running kasp genotyping reactions on the qiagen. Refer to the rotorgene q mdx instrument user manual for additional warnings, precautions, and procedures. Qiagen kit handbooks and user manuals are available at or can be. Please continue to use the older 36position and 72 position rotors with rotorgene 3000 instruments. Combination with the rotorgene q and qiagen realtime and 2. Qiagens realtime pcr cycler, the rotorgene q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Cfx96 biorad smartcycler with life science software 2. This screen appears when a new version of the rotorgene software has been installed and the rotorgene icon is double clicked for the first time after the installation. European launch of the new ce marked ipsogen calr rgq pcr kit to improve mpn diagnosis for leukemia patients in line with latest who recommendations and clinical guidelines. Guide to running kasp genotyping reactions on the qiagen rotor.
This is rotorgene q qiagen by labios on vimeo, the home for high quality videos and the people who love them. The countable reads are those that are used by the variant detection tool when calling the variant. Replicates run on the rotor gene hand pipetting full 36well rotor full 72well rotor. Alternatively, a computer in the assay setup room may be used for run setup. The newly released rotorgene 3000 uses a world first centrifugal design to provide the researcher with unparalled sensitivity and precision at a fraction of the costs associated with other high end systems. The easy nras kit is designed to be used with the instruments rotorgene q qiagen, rotorgene 6000 corbett research, cfx96 biorad, abi 73007500 applied biosystems and stratagene mx3000pmx3005p agilent technologies. Qiagen launches unique calr mutation assay for mpn diagnosis. Qiascreen hpv pcr test handbook instructions for use. Together with optimized qiagen kits for realtime pcr, the rotorgene q enables streamlined analysis for a wide range of applications. Rotorgene q pure detection 032011 the easytouse software provides. The rotorgene q uses a sophisticated heating and cooling design to achieve optimal reaction conditions. In addition to hrm channel, this pcr system has 5 color channels, green, yellow, orange, red and crimson. Developed over the past three years based on customer feedback worldwide, the software has been refined to provide an intuitive, wizard driven interface, enabling highest possible levels of flexibility and.
Qiagen kit handbooks and user manuals are available at. Rotor gene q qiagen real time pcr 5plex hrm system. The unique rotary format ensures optimal thermal and optical uniformity between samples which is critical for precise and reliable analysis. These products are not intended for the diagnosis, prevention or treatment of a disease. Kasp chemistry for allelic discrimination performs well on a qiagen rotorgene q instrument and this stepby step protocol will enable users to successfully setup, run and read plates on the qiagen rotorgene q. Print bookmark share for rotor gene q instruments using rotor gene q software version 2. Stateofthe art analyses supported by userfriendly software. Rotorgene screenclust hrm software is an extension to the rotorgene operating software. Rotorgene q is a program designed for the qiagen rotorgene q device that enables streamlined analysis for a wide range of applications. Page 124 userassigned label associated with the well. A rt pcr rotor gene was used for blue tongue diagnosis and the curve was absent either the internal control was absent, any suggestions. For all you need to run reactions using rotordiscs, choose the rotordisc 100 starter kit or the rotordisc 72 starter kit. The rotorgene 6000 real time dna amplification system is an open chemistry platform allowing the use of any real time chemistry. This user guide describes the features of the software.
Together with optimised qiagen kits for realtime pcr, the rotorgene q enables streamlined analysis for. This document is intended as a guide to running kasp genotyping reactions on the qiagen rotorgene q instrument. Alteration of incubation times and temperatures may result in erroneous or discordant data. Rotordiscs can be quickly and easily sealed with plastic film using a rotordisc heat sealer. You can perform manual reaction setup, or take advantage of qiagens automated solutions for. Qiagen rotorgene q cycler for realtime pcr and highresolution melting hrm applications by qiagen. Welcome to the rotorgene 6000 series software version 1. Rotorgene q pure detection 062010 comprehensive software and easy maintenance the easytouse software provides. Rotor gene q handbook readdownload the rotorgene multiplex rtpcr kit is designed for use with the rotorgene q and other rotorgene cyclers, providing ultrafast, highly reliable quantification. The qiagen rotorgene q is configured with a highresolution melting analysis hrm system, for more information and a wider range of applications. When used with optimized qiagen kits for realtime pcr, the rotorgene q can be used for a wide range of applications. This method uses a specially tuned, high intensity optical channel with a thermal resolution of 0. The software therefore aims to provide support for analysis of.
The rotorgene q qiagen real time pcr 5plex hrm system is configured with a highresolution melting analysis, for more information and a wider range of applications. The rotorgene offers the most userfriendly realtime analysis software system currently available. Rotorgene software manual franks hospital workshop. Rotor gene q software manual downloadqiagen rotor gene q user.
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